1H, 15N TROSY-HSQC (imino) spectrum of 5_SL6: 600 μM of 15N A/C/U and 13C 15N G labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D2O) measured at 298 K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50292
Reference: Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Info:
50 nt, MW: 16.41 kD, OD260nm: 462.8 1/mM
Sequence
GGACGUGGCU UUGGAGACUC CGUGGAGGAG GUCUUAUCAG AGGCACGUCC