1H, 15N HSQC (imino) spectrum of 5_SL4: 550 μM of U-15N and 13C G/C labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D2O) measured at 293K on a 800 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50347
Reference:
Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Bottaro, S. (2021) J. Am. Chem. Soc. preprint
Info:
44 nt, MW: 14.28 kD, OD260nm: 388.7 1/mM
Sequence:
GGGUGUGGCU GUCACUCGGC UGCAUGCUUA GUGCACUCAC GCCC