1H, 15N TROSY-HSQC (imino) spectrum of 5_SL6: 600 μM of 15N A/C and 13C15N G/U labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D2O) measured at 298 K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50351
Reference: Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Info:
46 nt, MW:14.86 kD, OD260nm 406.9 1/mM
Sequence:
GGCACGUCCA ACUCAGUUUG CCUGUUUUAC AGGUUCGCGA CGUGCC