RNA Results

¹H, ¹⁵N HSQC (imino) spectrum of 5_SL5stem: 150 μM of U-¹⁵N labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D₂O) measured at 298 K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB (doi:10.13018/BMR50340)

Reference: Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435

Info:

69 nt, MW: 22.33 kD, OD_260nm: 624 1/mM

Sequence:

GAUGGAGA GCCUUGUCCC UGGUUUCAAC GAUUCGUCGU UGACAGGACA CGAGUAACUC GUCUAUC

Sequence: GGGAUGGAGA GCCUUGUCCC UGGUUUCAAC GAUUCGUCGU UGACAGGACA CGAGUAACUC GUCUAUCCC