1H, 15N HSQC (imino) spectrum of 5_SL5A: 150 μM of U-15N labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D2O) measured at 298 K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50346
References:
Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Schnieders, R. (2021) Biomol. NMR Assign. published online
Bottaro, S. (2021) J. Am. Chem. Soc. preprint
Info:
33 nt, MW: 10.73 kD, OD260nm: 291.2 1/mM
Sequence:
GGCUGCUUA CGGUUUCGUC CGUGUUGCAG CC