RNA Results

¹H, ¹⁵N TROSY-HSQC (imino) spectrum of 5_SL2+3: 400 μM of U-¹⁵N labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D₂O) measured at 298K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50344

Reference:
Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Bottaro, S. (2021) J. Am. Chem. Soc. preprint

Info:

32 nt, MW: 10.37 kD, OD_260nm: 291.2 1/mM

Sequence:

GGAUCUCUUG UAGAUCUGUU CUCUAAACGA AC