RNA Results

¹H, ¹⁵N HSQC (imino) spectrum of 5_SL1: 150 μM of U-¹⁵N labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D₂O) measured at 298K on a 700 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50349

Reference:
Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435
Bottaro, S. (2021) J. Am. Chem. Soc. preprint
Richter, C. (2021) Biomol NMR Assign

Info:

29 nt, MW: 9.41 kD, OD_260nm: 257.4 1/mM

Sequence:
GGGUUUAUAC CUUCCCAGGU AACAAACCC