RNA Results

¹H, ¹⁵N TROSY-HSQC (imino) spectrum of 3_s2m: 388 μM of U-¹⁵N labeled RNA (25 mM potassium phosphate buffer pH 6.2, 50 mM KCl, 5% D₂O) measured at 298 K on a 600 MHz Spectrometer. Chemical shift assignment of Imino region is depicted and deposited in the BMRB doi:10.13018/BMR50341

Reference: Wacker, A., Weigand J.E. (2020) Nucleic Acids Res 48(22):12415-12435

Info:

45 nt, MW: 14.76 kD, OD_260nm: 412.1 1/mM

Sequence:

GGUUCACCGA GGCCACGCGG AGUACGAUCG AGUGUACAGU GAACC