1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5′-UTR of SARS-CoV-2

Biomolecular NMR Assignments (2021) 15:203–211

1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5′-UTR of SARS-CoV-2

R. Schnieders, S. A. Peter, E. Banijamali, M. Riad, N. Altincekic, J. K. Bains, B. Ceylan, B. Fürtig, J. T. Grün, M. Hengesbach, K. F. Hohmann, D. Hymon, B. Knezic, A. Oxenfarth, K. Petzold, N. S. Qureshi, C. Richter, J. Schlagnitweit, A. Schlundt, H. Schwalbe, E. Stirnal, A. Sudakov, J. Vögele, A. Wacker, J. E. Weigand, J. Wirmer-Bartoschek & J. Wöhnert

The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5′-untranslated region (5′-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5′-UUUCGU-3′ hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive ¹H, ¹³C and ¹⁵N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.