Protein Results

¹H,¹⁵N HSQC spectrum of Nsp9: 180 μM of U-¹⁵N labeled protein (25 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D₂O) measured at 298 K on a 700 MHz Spectrometer with chemical shift assignment depicted and deposited in the BMRB (entries 50621 & 50622, on hold)

Reference:
Dudás, E. F. (2021) Biomol. NMR Assign. Online ahead of print; Altincekic (2021) Front. Mol. Biosci.

 

¹H,¹⁵N HSQC spectrum of His₆-Nsp9: 230 μM of U-¹⁵N labeled protein (25 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, 2 mM TCEP, 0.02% azide and 5% D₂O) measured at 298 K on a 900 MHz Spectrometer.