1H,15N HSQC spectrum of Nsp3e: 616 μM of U-15N labeled His6-Nsp3e (20 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D2O) measured at 298 K on a 700 MHz Spectrometer.
1H,15N best-TROSY-HSQC spectrum of Nsp3e: 1.12 mM of U-15N labeled Nsp3e (20 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D2O) measured at 298 K on a 950 MHz Spectrometer with chemical shift assignment depicted and deposited in the BMRB (doi:10.13018/BMR50334)
Reference: Korn, S. (2020) Biomol. NMR Assign. 14:329–333; Altincekic (2021) Front. Mol. Biosci.