1H, 15N best-TROSY-HSQC spectrum of Nsp1-short: 11 μM of U-15N labeled protein (aa 13-128) (25 mM sodium phosphate buffer pH 7.0, 250 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D2O) measured at 298 K on a 950 MHz Spectrometer.
1H,15N -HSQC spectrum of Nsp1: 300-400 µM of U-15N labeled Nsp1 (50 mM sodium phosphate buffer pH 6.5, 200 mM NaCl, 2 mM DTT, 2 mM EDTA, 0.01 % v/v sodium azide, 0.001 % v/v DSS and 10% D2O) measured at 298 K on a 850 MHz Spectrometer with chemical shift assignment depicted and deposited in the BMRB (entry 50620, on hold)
Reference: Wang, Y. (2021) Biomol. NMR Assign. Online ahead of print ; Altincekic (2021) Front. Mol. Biosci.