1H, 15N best-TROSY-HSQC spectrum of Nsp1-short: 11 μM of U-15N labeled protein (aa 13-128) (25 mM sodium phosphate buffer pH 7.0, 250 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D2O) measured at 298 K on a 950 MHz Spectrometer.
1H, 15N best-TROSY-HSQC spectrum of His6-Nsp1-long: 98 μM of U-15N labeled protein (aa 1-180) (25 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, 2 mM TCEP, 0.02% azide and 10% D2O) measured at 298 K on a 700 MHz Spectrometer.